Wheat retrotransposon families identified by reverse transcriptase domain analysis.

نویسندگان

  • Y Matsuoka
  • K Tsunewaki
چکیده

Variations in the reverse transcriptase domain were analyzed to clarify the family structure of retrotransposons in the wheat genome. Seven families were identified on the basis of the nucleotide sequence similarities of the domain. These families are common to the genomes of ancestral diploid species of wheat, evidence that they were established prior to diploid speciation, and their presence in barley and rice was suggested. Recent activity of some of the families was inferred in wheat on the basis of data that included synonymous and nonsynonymous nucleotide substitution rates in the reverse transcriptase domain.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Characterization of Ty1/copia-like retrotransposon families from pigeonpea genome.

Retrotransposons contribute significantly to the size, organization, and genetic diversity of their host genomes. To characterize novel retrotransposon families in pigeonpea and develop retrotransposon-based sequence-specific amplification polymorphic markers, in silico homology sequence search was carried out against the whole genome shotgun sequence of pigeonpea variety Asha (ICPL87119). For ...

متن کامل

A superfamily of Arabidopsis thaliana retrotransposons.

We describe a superfamily of Arabidopsis thaliana retrotransposable elements that consists of at least ten related families designated Ta1-Ta10. The Ta1 family has been described previously. Two genomic clones representing the Ta2 and Ta3 elements were isolated from an A. thaliana (race Landsberg erecta) lambda library using sequences derived from the reverse transcriptase region of Ta1 as hybr...

متن کامل

Activation of Ty1-copia Group Retrotransposons of Dendrobium officinale under Abiotic Stress Conditions

By using universal primer Ty1-copia retrotransposon reverse transcriptase (RT), the conserved reverse transcriptase domain of about 260 bp, which was induced by cold stress and osmotic stress, was amplified by RTPCR from the Dendrobium officinale in this study, indicating that the retrotransposon could be activated by stress conditions. The amplicons were recovered and cloned for sequencing and...

متن کامل

Role of integrase in reverse transcription of the Saccharomyces cerevisiae retrotransposon Ty1.

Reverse transcriptase (RT) with its associated RNase H (RH) domain and integrase (IN) are key enzymes encoded by retroviruses and retrotransposons. Several studies have implied a functional role of the interaction between IN and RT during the replication of retroviral and retrotransposon genomes. In this study, IN deletion mutants were used to investigate the role of IN on the RT activity of th...

متن کامل

Divergent long-terminal-repeat retrotransposon families in the genome of Paragonimus westermani.

To gain information on retrotransposons in the genome of Paragonimus westermani, PCR was carried out with degenerate primers, specific to protease and reverse transcriptase (rt) genes of long-terminal-repeat (LTR) retrotransposons. The PCR products were cloned and sequenced, after which 12 different retrotransposon-related sequences were isolated from the trematode genome. These showed various ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Molecular biology and evolution

دوره 13 10  شماره 

صفحات  -

تاریخ انتشار 1996